Gene expression is central to cell biology. Disease pathways often involve changes in the expression levels of at least some genes. RNA-seq has become one of the most popular experimental methods to quantify gene expression levels. This 2-day, hands-on workshop will provide an introduction to a typical bulk RNA-seq protocol and focus on the data analysis steps for recovering actionable insights.
You’ll learn how to perform a quality check of the raw data in the typical format provided by sequencing centers, process it by trimming adapters, map trimmed reads to a reference genome, and tally gene-wise counts. You’ll learn to analyze high-throughput data, learn about various methods of quality control at each step, and interpret data formats such as FASTQ, BAM, and GFF.
This is an introductory workshop in the RNA-Seq Analysis series.
Prerequisites for demo: Participants should have familiarity with Unix and be comfortable running commands on the command line.
Important: Visit the workshop site for more details and materials.
