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Grugliasco

Largo Paolo Braccini 2

10095 Grugliasco

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EMPHASIS project (Effective Management of Pests and Harmful Alien Species – Integrated Solutions) aims to ensure a European food security system and the protection of biodiversity and of ecosystem services while developing integrated mechanisms of response measures (practical solutions) to predict, to prevent and to protect agriculture and forestry systems from native and alien pests threats.

The reduction in available plant protection products and the requirement of EU Member States to promote low pesticide input and implement IPM principles, as a result of EU hazard-based legislation, is a key driver for the development of alternative and sustainable pest strategies. However, the key to attaining greater uptake of alternative strategies is through providing products or methodologies that can perform as well as traditional pesticides, whilst at the same time providing the same ease of use and cost effectiveness.

The overall goal of EMPHASIS is to develop and promote the take-up of practical and effective solutions to prevent and protect European crops from native and alien pest threats, improving productivity and resilience of European agriculture, in the context of climate change.

The course is made up of three sections:

  • innovative strategies for diagnosis and management in horticultural crops;
  • emerging pests in horticultural crops;
  • use of LAMP technology.

This course aims to train young researchers on epidemiological issues related to emerging pests and pathogens, and on innovative management strategies in horticultural systems. It also aims to provide skills about the use of innovative and effective diagnostic systems.

Molecular methods based on DNA analysis are widely used in plant disease diagnostic, due to their high efficiency, sensitivity, specificity and rapidity for early detection and identification of a broad range of plant pathogens and pests. Loop-mediated isothermal amplification (LAMP) is an emergent molecular tool offering rapid, accurate, and cost-effective diagnosis of plant diseases. LAMP assay enables to use fast DNA extraction methods suitable for on-site application. In addition this assay uses an enzyme tolerant to chemical inhibitors often found in environmental samples, making this method an excellent tool for the in-field detection of plant pathogens.

The purpose of the course is to familiarize trainees with the basic principles of LAMP as diagnostic tool and to train them on in-field DNA extraction methods and common instruments/reagents of a LAMP assay. The course will provide trainees the basis for designing, performing and troubleshooting a LAMP assay, according to EPPO Standard PM 7/98.

Training about LAMP technology will be structured on two levels (Basic and Advanced) to address different training needs of participants.

The Basic Course will be held in Italian language.

The full Summer School program can be downloaded at the following link.

http://www.emphasisproject.eu/latest_news_dett.php?id=28

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Grugliasco

Largo Paolo Braccini 2

10095 Grugliasco

Italy

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