Tips and Tricks to Help Develop and Run Microplate-based Assays

Today’s scientists rely on both biochemical and cell-based in vitro assays for their research. Critical parameters for achieving success with these assays include understanding the assay dynamics, the instrumentation involved in reading the assay, and, in the case of cell-based assays, understanding how the environment in which the cells are grown impacts cellular physiology. Factors such as microplate geometry, density, surfaces, and instrumentation all play important roles in the success of the assay. In this seminar, we will discuss ways to enhance your assay performance. Topics to be discussed include:

• Microplate selecRon and its role in the success of the assay

• Important consideraRons for liquid handling

• The importantance of Rps to your overall assay

• Choosing the correct Rp for your liquid handling needs

• The importance of choosing the correct instrumentaRon and se`ngs to run your assay

Speaker

Mark Rothenberg Ph.D.
Manager ScienRfic Training and Education, Corning

Mark Rothenberg graduated from Emory University with his PhD in Cell and Developmental Biology. Over the
past 25 years Mark has held posiRons in both Academia and industry where he has developed an experRse in
the areas of assay development, cell culture (mulRple types including primary lines), and cell culture scaleup.
Prior to his current posiRon as Manager ScienRfic Training and EducaRon he worked and led the
applicaRons team.