Four-part course:

Saturday, October 18, 2:00pm-6:00pm EDT

Sunday, October 19, 2:00pm-6:00pm EDT

Monday, October 20, 6:00pm - 9:00pm EDT

Sunday, October 26, 2:00pm-6:00pm EDT

CRISPR/Cas9 is one of the most transformative recent developments in biotechnology, radically simplifying the process of editing genomes and increasing its accessibility. Want to learn how to do hands-on genome editing?

In this class you will:

Learn how to culture and work with Saccharomyces cerevisiae (brewers yeast)
Assemble a plasmid encoding a CRISPR/Cas9-mediated gene deletion
Generate a repair template encoding a fluorescent protein that will be inserted into the yeast genome
Use the constructs you produce to transform yeast and confirm the results by DNA sequence analysis
Discuss emerging technologies and applications of CRISPR in other organisms

Prerequisites:

You must be familiar with molecular biology lab techniques such as pipetting, gel electrophoresis, use of restriction enzymes and PCR (Genspace Biohacker Boot Camp or equivalent lab experience)

COVID-19 Safety Notice:

If you are feeling unwell, suspect that you have been exposed to COVID-19 or have tested positive in the past 7 days, please do not attend and let us know ASAP (info@genspace.org). If you cancel after our 7-day policy, we cannot refund your ticket, but we can exchange and offer credits toward future classes. If you have signed up for Biohacker Boot Camp, we will automatically transfer your registration to the next month’s dates unless you tell us otherwise.

Genome Editing with CRISPR-Cas9