Bio-Trac’s Thursday Seminar Series – Have lunch with us.
Each Thursday: April 30 – June 18, 2020 from 12:15-12:45
Special Topics in Biotechnolgy

Featuring active research scientists from respected institutions.
Open to the Scientific Community - Registration is Required

April 30, 2020
Dr. Serguei Kozlov (NIH/NCI)
Useful Tips on Designing Successful CRISPR Experiments
This presentation will summarize the main molecular features and key applications of CRISPR/Cas9 genome editing technology. Representative examples of CRISPR experimental strategies will be discussed and some practical advices shared on how to design efficient CRISPR systems to achieve gene disruption or more subtle targeted alterations and insertions of desired DNA sequences.

May 7, 2020
Dr. Allissa Dillman (NIH/CIT)
R - Harnessing the Power of the Tidyverse
This walk through presentation will demonstrate the ability to generate multiple plots with a few simple commands.

May 14, 2020
Dr. Fatah Kashanchi (GMU)
Separating Viruses from Exosomes
Dr. Kashanchi will discuss the separation of virus away from exosomes using multiple models of virus infection including HIV-1. Functional analysis of the exosomes will be discussed on recipient uninfected cells along with mechanisms of how these exosomes can control the immune cells by either cell death or reprogramming. Finally, he will discuss the latest findings on how to separate Corona viruses away from exosomes since they have similar sizes coming out of infected cells. This will aid in better understanding of the cytokine storm observed in patients post infection.

May 21, 2020
Dr. Elke Bergmann-Leitner (WRAIR)
Rare Cell Detection by Flow Cytometry: Enables in-depth Characterization of Functional Lymphocyte Subsets

This presentation will describe the importance of sample size calculation for flow cytometric analysis and limitations of conventional flow cytometry regarding detection threshold and sensitivity. We will also discuss methods enabling rare cell detection and characterization of low frequency cell populations.

May 28, 2020
Dr. Richard Lee (JHU)
Bisulfite Pyrosequencing: Quantitative Approach for Assessing DNA Methylation

There continues to be much interest in epigenetics as one of the primary mechanisms that underlie numerous physiological and pathological processes, as well how organisms interact with the environment. In this webinar, I will cover the basics of bisulfite pyrosequencing, which is a highly quantitative method for measuring DNA methylation. I will also provide a brief overview of DNA methylation and use examples from my work to demonstrate how pyrosequencing can be applied in biomedical research.

June 4, 2020
Dr. Kristen Bircsak (Mimetas)
Modeling the Prostate Tumor Microenvironment using Organ-on-a-Chip Technology

Many of the current two-dimensional (2D) cell culture models used in prostate cancer research fail to recapitulate the prostate tumor microenvironment leading to low predictivity of the models. Physiologically based, 3D in vitro systems offer the potential to enhance the complexity and fidelity of 2D systems as they incorporate many components present in the in vivo tumor including extracellular matrix, stromal cell types, and perfusion. This presentation will review the setup and applications of a prostate tumor-on-a-chip model.

June 11, 2020
Immuno-Oncology Multiplexing and Hyperplexing

June 18, 2020
Dr. Maria Hernandez (NIH/NCI/FNL)
Sample Processing Considerations for Single Cell Sequencing – A Crucial Component of Experimental Design and Data Interpretation

A successful single cell study starts with a thoughtful consideration of how to prepare a cell or nuclei suspension. Choices on how to prepare sample may be intricately related to tissue type, study design and the experimental questions sought after. Knowledge of how samples were processed, including any enrichment or selection can influence data interpretation in important ways.

All the best

Mark Nardone, PMP
Director, Co-Founder
Bio-Trac® Training Programs
mark.nardone@biotrac.com

240-567-2159 (Office)
301-802-7708 (Cell) - Use this #, currently working remotely

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Bio-Trac's Thursday Seminar Series – Special Topics in Biotechnology