On behalf of the "Chaire en Prévention et Traitement du Cancer", with endorsement by the Canadian Cytometry and Microscopy Association (CCMA), Expert Cytometry and MiaCellavie are pleased to announce a 2-day Advanced Experimental Design flow cytometry training course in Montreal.

Schedule

October 24th/ Day 1:

09:00-09:30 Introduction and Course Objectives

09:30-10:30 Generating Data: Refresher on Flow Cytometry

10:30-10:45 Break

10:45-11:30 Keeping it real: Controls in Cytometry.

11:30-12:00 For fun and profit: Theory and Practice of Complex Compensation

12:00-13:00 Lunch

13:00-13:45 How it’s made: Theory and Consideration of Panel Design

13:45-14:15 Make it real: Panel Design using Fluorish

14:15-14:45 And now introducing: Review of the Experimental Dataset

14:45-15:00 Break

15:15-16:15 Make it work: Experimental Optimization of Developed Panels

16:15-17:00 Lab1: Titration Data Analysis (Data set 1)

October 25th/ Day 2:

09:00-09:30 Voltage Optimization analysis walkthrough (Lecture with Flowjo)

09:30-10:30 Lab 2: Individualized Voltage Optimization analysis

(Students work through data sets on their own with Instructor support)

10:30-10:45 Break

10:45-11:15 Analysis walkthrough (Lecture with Flowjo)

11:15-12:00 Lab 3: Individualized Analysis – Compensation in FlowJo

(Students work through data sets on their own with Instructor support)

12:00-13:00 Lunch

13:00-14:00 Lab 4: Individual Analysis – Establish and refine analysis

(Students work through data sets on their own with Instructor support)

14:00-15:00 Show what you mean. Practical Statistics and publishing your data

15:00-15:15 Break

15:15-15:45 Forensic Flow Cytometry (Lecture with FlowJo)

15:45-16:45 Lab 4: Forensic Flow Cytometry

(Students work through data-sets on their own with Instructor support)

16:45-17:00 Final Questions