West Coast CrawFly Invertebrate Neurophysiology Course
Claremont, CA, United States
ADInstruments and the Department of Neuroscience at Pomona College will co-host a hands-on workshop for higher education life science instructors seeking to expand their curriculum reach in the neurosciences. The training event will be based on topics explored in the “Crawdad Project,” a three-year program funded by the National Science Foundation to promote the use of invertebrates in undergraduate physiology and neuroscience lab courses. The goals of this hosted workshop are to provide teachers hands-on experience with invertebrate preparations that demonstrate basic principles of nervous system physiology, and can easily be incorporated into laboratory courses back home.
CRAWDAD (3 1/2 Days)
- Motor innervation
- Sensory systems
- Ionic bases of resting and action potentials
- Synaptic transmission
- Synaptic plasticity
- Central pattern generation for rhythmic motor activity
Exercises to be covered:
• Passive Membrane Properties - Introduction to membrane properties and equipment use
• Crayfish Motor Nerve Recording - Extracellular recording of spontaneous and reflex motor activity
• Ionic Basis of Resting Potential - Intracellular recording of crayfish muscle
• Ionic basis of action potential - Intracellular recording from snail brain neurons
• Plant Action Potential - Intracellular recording of a large alga cell that fires an AP
• Synaptic Connectivity - Simultaneous Extracellular and Intracellular recording from nerve and muscle; matching PSP’s with AP’s
• Synaptic Plasticity - Stimulating a nerve, recording muscle synaptic potentials; facilitation and PTP
• Stretch Receptor - Extracellular recording of crayfish stretch receptor • Conduction velocity measurements from multiple motor neurons simultaneously; rhythmic motor pattern generation
Skills to be covered:
• Neuromuscular Intro - Preparations used in Crayfish Motor Nerve, Resting Potential, Axonal conduction velocity; Rhythmic Motor Activity and Synaptic labs
• Dissecting Tips - General advice on successful dissection
• Recording Tips - Background and troubleshooting for extra- and intracellular computer based data acquisition • Data Analysis - including spike sorting, matching presynaptic action potentials with postsynaptic potentials; measuring AP conduction velocity; calculating time constants for sensory receptor adaptation
FRUITFLY (1/2 Day)
- Use of the Drosophila electroretinogram (ERG) recording as a neural signal in the fly eye in response to flashes of light
- Drosophila ERG response variants as a test of sensitivity to light stimulation including intensity, duration and wavelength as a framework for student analysis
- Affects of mutations on key characteristics of intracellular signaling, synaptic transmission or neuronal function as it correlates directly to Drosphilia ERG waveforms variance
- Acquisition of wild type and mutant ERGs and the student exploration between genetics, biochemical pathways, and electrophysiology
The final course agenda will be made available beginning the first day of the course and is dependent on instructor resources and input from attendees. If there is a specific topic you would like to cover outside of this schedule, one-on-one coaching sessions will be available. The outline above is representative of the standard topic covered but is subject to change.
*Course cancellations must be received prior to 30 days before the start date of the event to receive a full refund. No-show's and late cancellations will be charged the full event price.
Lead Course Instructor
Senior Research Associate at Cornell
Dr. Bruce Johnson is currently a Senior Research Associate with the Harris-Warrick Lab in the Department of Neurobiology and Behavior at Cornell University. Bruce under the guidance of Dr. Ron Hoy hosted the first series of NSF-funded Crawdad workshops and has an extensive background in neurobiology research and education from his past and current affiliations with the Marine Biology Laboratory, Shoals Marine Lab, PKAL and FUN. He is a lead instructor at the annual Crawdad and CrawFly invertebrate neurobiology educator workshops hosted at Cornell.
When & Where
ADInstruments has been designing and manufacturing PowerLab data acquisition systems for life science since 1988. Our goal is simple: deliver a data acquisition solution that is high quality, easy to use and perfectly suited for its many intended applications in corporate and academic research, and higher education.
ADInstruments, Inc. organizes training courses and events for researchers and educators in North America.