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Two Days of Flow Cytometry Training - Cleveland

Tim Bushnell, Ph.D.; Director of the URMC Shared Resource Labs

Monday, December 5, 2016 at 8:30 AM - Tuesday, December 6, 2016 at 5:00 PM (EST)

Two Days of Flow Cytometry Training - Cleveland

Ticket Information

Ticket Type Sales End Price Fee Quantity
Day 1 : Priciples (only) Ended $275.00 $16.12
Day 2 : Polychromatic (only) Ended $275.00 $16.12
Two Day Course Ended $425.00 $24.37

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Event Details

DAY 1 : Principles of Flow Cytometry

 


Educational Objectives 

This one day course will cover the five fundamental areas critical for successful flow cytometry:  Reagents, Hardware, Controls, Compensation, and Data Analysis.  At the conclusion of the course, attendee’s will have a better understanding how these different aspects interact so they will be able to obtain the highest quality data possible. 

Target Audience 

Users with basic flow cytometry knowledge seeking to understand the essential concepts in flow cytometry and a desire to move into higher-end assays (from 4 color to 8+ color edperiments).  Learning these principles will ensure your flow cytometry experiments will yield results – ultimately saving you time and money in the long run.

Note: A computer with analytical software is helpful but not critical.

Day 1 Agenda 

09:00-09:30              Course Introduction and Activity 1 – Design the Experiment

09:30-10:15              Lecture 1 – Software (Reagents, Fluorescence and Fluorochromes)

10:15-10:30              Introduction to Spectral Viewers

10:30-10:45              Coffee Break and questions

10:45-11:30              Lecture 2 – Hardware (Fluidics, Optics and Electronics)

11:30-12:15              Lecture 3 – Compensation Principles and Best Practices

12:15-13:15              Lunch and Sponsor Talk

13:15-13:45              Activity 2 – Compensation practice

13:45-14:30              Lecture 4 – Controls in Flow Cytometry

14:30-15:15              Lecture 5 – Understanding Flow Cytometry Data

15:15-15:30              Coffee Break and questions

15:30-16:15              Lecture 6 – Rare Event Analysis

16:15-16:45              Activity 3 – Test yourself

16:45-17:00              Final Questions 

 

 

 


 

DAY 2 : 10 to 18 Color Panel Design

 


Educational Objectives 

This one-day seminar will go through the steps necessary to develop and implement 10+ color flow cytometry panels. Beginning with the design of a panel, we move through the critical steps of instrument optimization, titration, and voltage determination using real-world data. We will work through the standardization of the signals over time, optimizing data analysis while emphasizing the use of critical controls. At the end of the class, attendees will have the necessary skills to build new and improve existing polychromatic flow cytometry panels.

Target Audience 

Researchers with more than 6 months experience and who are seeking to expand their current flow cytometry panels into 10+ colors.

Note: A computer with analytical software is helpful but not critical.

Day 2 Agenda 

09:00-09:15              Course Introduction

09:15-10:00              Lecture 1 – Hardware Optimization for Polychromatic Panel Design

10:00-10:30              Lecture 2 – Brightness and Flurochromes

10:30-10:45              Coffee Break and questions

10:45-11:30              Lecture 3 – Theory of Panel Design

11:30-12:00              Lecture 4 – Automatic in Panel Design

12:00-13:00              Lunch and Sponsor Talk

13:00-14:00              Activity  – Design a polychromatic Panel

14:00-14:30              Review of student designed panels

14:30-15:30              Lecture 5 – Optimization and Standardization of Polychromatic Panels

15:30-15:45              Coffee Break and questions

15:45-16:45              Lecture 6 – Statistics in Flow Cytometry

16:45-17:00              Final Questions

Have questions about Two Days of Flow Cytometry Training - Cleveland? Contact Tim Bushnell, Ph.D.; Director of the URMC Shared Resource Labs

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When & Where


Lerner Research Institute

Cleveland, Ohio

Monday, December 5, 2016 at 8:30 AM - Tuesday, December 6, 2016 at 5:00 PM (EST)


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Organizer

Tim Bushnell, Ph.D.; Director of the URMC Shared Resource Labs

Tim is an expert flow cytometrist and technical applications scientist. His goal is to help researchers get the best possible data out of their flow cytometry experiments as possible. He is the director of one of the largest medical research cores in the world and is the co-founder of Expert Cytometry. Tim's specialities include flow cytometry, cell sorting, design of polychromatic panels, data analysis, quality control, core management operation, training, and education. Here is a brief history of Tim's appointments:

2012, Appointed as Director of the URMC Shared Resource Labs.
2011, Appointed Assistant Professor of Pediatrics and Hematology.
2008, Appointed Research Assistant Professor and Director of the URMC flow cytometry core.
2004, Founded the WNYFUG group, served on the board of GLIIFCA, currently serving on the Executive Committee of ISAC since 2008, currently the Secretary and co-chair of the Membership Services committee.
2003, Appointed the Director of the Center for Pediatric Biomedical Research’s Flow Cytometry Facility at the University of Rochester Medical Center.

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Two Days of Flow Cytometry Training - Cleveland
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