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Troubleshooting Your Data

From sample preparation to data analysis, there are many places where small errors can dramatically impact your data.

  • Counting your cells at the beginning and end of the staining procedure is critical to determine sample loss because of preparation process. Learn ways to minimize these losses.
  • What three steps in sample prep are critical to ensure a high-quality single cell suspension suitable for staining?
  • Loss of resolution caused because of non-specific binding compromises sensitivity. There are several tweaks to existing protocols that can reduce the NSB. Learn what those tweaks are.
  • Compensation, if performed incorrectly, can lead to loss of resolution. Understanding the three rules of compensation and how to recognize when they have been violated will reduce problems in your data analysis.
  • Placing gates is a critical step in data analysis. Since gating is an all-or-nothing prospect care must be taken to ensure they are placed properly. Understanding the tools needed to properly place and verify gating choices will help ensure this critical step doesn’t compromise your data.
  • Speaking of sensitivity, the goal of statistical analysis is to identify differences between the controls and treatments. Discover how to apply statistics properly from the start of the experiment to the end.
  • Quality control of the flow cytometer is run to ensure the system is running optimally. Learn how to use the QC data for identifying problems in acquisition.

At the conclusion of this webinar, attendee’s will have a better understanding of issues that can arise in their flow cytometry experiments and be able to find the solutions to prevent these issues or in some cases salvage the data.

*This webinar will be recorded and sent to you if you join and can't make it to the live event.

The success or failure of an experiment can depend on the quality and performance of the instrument being used. Learn about the best controls to know how well the machine is performing before starting your an experiment. The Fluorescence Minus One control (FMO) is discussed in many papers as a critical control. Understanding on how to use the FMO control and how it impacts the analysis of the data is essential.

Do you still rely on Isotype controls for setting gates? Do you understand what information the Isotype control can provide and how to prevent over analysis of this control? When performing experiments over time, what additional controls should be integrated into the workflow? Understand what these controls tell the researcher about how the experiment is changing over time. Have you heard of the isoclonal control? What does this control tell you about your antibodies and cells? Learn where this control is useful for your experiments.

This webinar will review the two major classes of controls used in any flow cytometry experiment - instrument specific controls and experiment specific controls. We will also discuss how each of these controls play a role in properly interpreting your experiment.

Purchasing an Eventbrite ticket will give you exclusive access to the this seminar, which is hosted by WebEx, and will allow you to interact directly with the workshop instructors by asking questions using WebEx's Q&A tool. After the seminar, you will also be given a link to download a video of the course for free to watch later.

An hour before the seminar (12PM EST), you will receive an access link via email that will allow you to join the seminar on WebEx, a seminar hosting server. The seminar starts at 1PM EST. ***Your ticket is also your receipt.

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