$250

SoCal Flow Summit: Antibody Panels, Cycles, and Apoptosis

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SoCal Flow Summit

University of California Irvine

Stem Cell Research Center

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Description

Course Objectives: Flow cytometry is a powerful tool for exploring the state of cells at the single cell level. This one-day course will cover the principles of phenotyping (panel design), cell cycle analysis and apoptosis.

Target Audience: Any researcher using flow cytometry for their experiments seeking to improve the ability to design polychromatic panels, and build complex analytical experiments combining different assays. Recommended for those with at least 6 months of flow experience performing 4+ color flow cytometry experiments

Agenda

09:00-09:15 Course Welcome and Instructor Introduction

09:15-10:00 Panel 01:Generating good data

10:00-10:30 Panel 02:Fluorchromes, brightness and detection

10:30-10:45 Coffee Break and questions

10:45-11:30 Panel 03: Principles of panel design

11:30-12:00 Panel 04: Automation of panel design

12:00-13:00 Lunch

13:00-14:00 Cell Cycle 01: Principles of cell cycle analysis

14:00-15:00 Cell Cycle 02: Cell cycle data analysis

15:00-15:15 Coffee Break and questions

15:15-16:15 Apoptosis 01: Principles of apoptosis

16:15-16:45 Apoptosis 02: Apoptosis data analysis

16:45-17:00 Wrap-up and questions

Note: A personal computer is useful, but not critical for the course.

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SoCal Flow Summit

University of California Irvine

Stem Cell Research Center

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