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Mahley Auditorium, Gladstone Institutes

Genentech Hall, N-114 (Training Session)

San Francisco, CA 94158

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During this one-day symposium, we will cover techniques, platforms and methods that are used in analyzing single cell data. The symposium has two sessions: a series of seminars in the morning and hands-on workshops in the afternoon. The morning session will start with the introduction of single cell platforms, followed by talks focusing on applications of various technologies to diverse research areas. In the afternoon, we will have three consecutive hands-on sessions with increasing difficulties, ranging from how to use Cell Ranger and Seurat to advanced analyses beyond these common tools.

Symposium Agenda

Light Breakfast 8:30 - 9am

Morning lecture session 9am - 12:30pm
(Mahley Auditorium, Gladstone Institutes, 1650 Owens St.)

  • Introduction to single cell sequencing technologies - Eric Chow, PhD. (9-9:45 am)
  • Leveraging machine learning & AI in the analysis and interpretation of Tapestri single-cell DNA data - Manimozhi Manivannan, PhD. (9:45-10:15 am)
  • Break (15mins)
  • Dissecting the heterogeneity of pluripotent stem cell multicellular systems with single cell RNA-seq - Todd McDevitt, PhD. (10:30-11:00)
  • Revealing the full complexity of biology at single cell scale - Chris Rutherford, PhD (11:00-11:30)
  • Break (15min)
  • Multiplexed single cell RNA-sequencing -Jimmie Ye, PhD. (11:45-12:30)

Lunch Break 12:30 - 2pm

Afternoon Hands-on Sessions 2 - 6pm
(Genentech Hall, Room N114, 600 16th St)
Laptop and familiarity with R required

Session 1: 10x Genomics initial data analysis - Cell Ranger overview and Loupe Cell Browser visualization - Rea Dabelic, PhD
Break (5min)
Session 2: Introduction to single cell RNA-seq analysis with Seurat - David Joy
Break (5 min)
Session 3: Analysis beyond Seurat

  • Normalization and differential expression - Reuben Thomas
  • Network visualization and analysis of single cell datasets in Cytoscape - Alex Pico and Scooter Morris
  • Fast and scalable cell type atlas search with the CellFishing package - Dan Fulop

Date and Time

Location

Mahley Auditorium, Gladstone Institutes

Genentech Hall, N-114 (Training Session)

San Francisco, CA 94158

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