RNA-seq analysis: from reads to differentially expressed genes.

Lead trainer:

Tristan Reynolds (Melbourne Bioinformatics, The University of Melbourne)

Workshop Description:

Across 2 days :

Wednesday, 25 June -10:00am - 2:00pm
Thursday, 26 June - 10:00am-2:00pm

Attendees can expect to gain an understanding of approaches to and practical experience in: quality control of short reads, alignment, gene counts, data normalisation, differential expression and visualisation. Places are limited to encourage discussion and experienced RNA-seq and Galaxy trainers will be present to answer your questions.

This training workshop will be conducted using Galaxy and the workshop materials are based on the following Galaxy training workshops:

It is important that you are familiar with navigating the Galaxy platform as the workshop will not include introductory content on how to use the platform.

Learning objectives:

By the end of this training, participants will be able to:

Perform quality control of short read data
Align reads to a reference genome and generate gene counts
Convert gene counts to differentially expressed genes and normalise data
Visualise differential gene expression

Target audience:

Researchers who have, or will soon acquire, RNA-seq short read data that they need to analyse themselves. No programming experience is required.

Eligibility:

This free workshop is available to staff and students at The University of Melbourne and its affiliated institutes.

When registering for this event you must provide an email address for an affiliated institution, or your registration may be cancelled.

Prerequisites and Requirements:

No programming experience is necessary, but familiarity with navigating the Galaxy platform is required as this workshop will not include introductory content on how to use the Galaxy platform. Attendance at a previous Galaxy workshop or working through this material is sufficient for attendance.

This is an in person hands-on workshop and attendees must bring their own computers (laptop chargers also recommended) with access to the Uniwireless or Eduroam internet/wifi. The following is also required:

Web browser (Firefox or Chrome recommended)
Participants should register for a Galaxy Australia account using their institutional email address at least 3 days prior to the workshop

For University of Melbourne participants: If you do not receive an activation email within 2 hours of registering in your Inbox or Junk Email folder, the activation email may be held by the Mimecast Email Security and you will need to "release" the email for it to appear in your Inbox. You can login to the Mimecast Email Security Portal using your University of Melbourne email and check/release held emails here: https://uom.login-au.mimecast.com/u/login/?gta=portal#/login

Access

If you require any further information, or have any access requirements in order to participate in this workshop, please contact us as soon as possible to discuss your requirements:

We recommend following our eventbrite page if you wish to be alerted when we release new workshops for registrations. If you require any further information, please contact Melbourne Bioinformatics at:

bioinformatics-training@unimelb.edu.au

RNA-seq : from reads to differentially expressed genes using Galaxy