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RNA-Seq Differential Gene Expression Analysis Made Simple

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Yawkey Conference Center

450 Brookline Avenue

Smith Buliding - SM308/309

Boston, MA 02215

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Description

This free hands-on workshop will walk participants through a RNA-Seq differential gene expression analysis from raw sequence files (fastq files) to gene set enrichment analysis using infrastructures developed by the CCCB and general analysis resources available to the community. Participants will perform and learn:

  • How to align and quantify mRNA-Seq raw sequence files using CCCB cloud pipeline
  • How to perform differentially gene expressed analysis
  • How to perform Principle Component Analysis, Clustering, and pathway enrichment analysis using WebMeV
  • How to perform Gene Set Enrichment Analysis using Broad GSEA with CCCB cloud pipeline output

This workshop aims to enable experimental and clinical scientists with no computational background to quickly and conveniently perform simple RNA-Seq analysis from raw sequence data. Participants are expected to bring their own laptop and are encouraged to prepare a small number of their own sequence data for analysis. Space is limited, RSVP is required.

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Date and Time

Location

Yawkey Conference Center

450 Brookline Avenue

Smith Buliding - SM308/309

Boston, MA 02215

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