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Optimized target detection in qPCR [SS4-0418]

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AULA RAMON ARECES, Centro de Biología Molecular “Severo Ochoa”

UAM

Madrid

Spain

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Optimized target detection in qPCR: new probe and primer chemistries for gene expression and genotyping


qPCR is a primary method to examine gene expression and genotyping, but there continues to be a need to improve the sensitivity and specificity of qPCR-based methods. The discussion will focus on how to:

• Increase the sensitivity of qPCR assays with internally quenched, 5’ hydrolysis PCR probes.

• Improve PCR specificity in gene expression and allelic discrimination studies with a new RNase H2 enzyme-mediated PCR method.


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AULA RAMON ARECES, Centro de Biología Molecular “Severo Ochoa”

UAM

Madrid

Spain

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