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Lunch & Learn: New exosome isolation method and its applications

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FUJIFILM Diosynth Cambridge Collaboration Center (C3)

One Kendall Square, Binney St

Building 400

Cambridge, MA 02139

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Lunch & Learn: New exosome isolation method and its applications

Exosomes are in the spotlight of drug delivery research, diagnostic and new therapy development. Once thought to be merely carriers of unnecessary cell content, exosomes are now known to regulate cell-cell communication, transporting lipids, proteins, and RNA. Isolating exosomes can be, however, cumbersome, and traditional centrifugation methods often result in poor yield and low purity.

Addressing these needs in life science research, FUJIFILM Wako has developed a novel method for isolating Exosome by using Tim-4/PS affinity method. In this seminar, Wako introduces its MagCapture™ product line, as well as scientific findings by using this technology.

Participants will have opportunities to;

  • Learn how MagCapture™ Exosome Isolation Kit enables isolation and purification of exosomes with four simple steps

  • See case studies demonstrating the data obtained using MagCapture™ products

  • Join in a user community for discussion and Q&A


11:30-12:00

Registration


12:00-12:40

Extracellular vesicles (EVs) from human bone marrow mesenchymal stem cells (hBM-MSCs) repair organ damage caused by cadmium (Cd) poisoning in a medaka model

Presenter:

Tomoko Obara, Ph. D.
Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA

Abstract:

Because chronic exposure to heavy metal is undiagnosed in the early stages, the chelation therapy is ineffective. EVs derived from stem cells have been demonstrated to reduce disease pathology in both acute and chronic kidney disease models. To test the ability of EVs derived from hBM-MSCs to treat Cd damage, we generated a Cd-exposed medaka model. This model develops Cd-induced cell damage of various organs and tissues, and shows decreased survival. Intravenous injection of highly purified EVs from hBM-MSCs repaired the damage to kidney proximal tubules, glomerulus podocytes, repaired bone deformation, and improved survival. In addition to the Cd injury repair, our system also serves as a model with which to study age- and sex-dependent cell injuries of organs caused by other various agents and diseases. The beneficial effects of EVs on the tissue repair process, as shown in our novel Cd-exposed medaka model, may open new broad avenues for interventional strategies.


12:40-13:00

Characteristics on PS Affinity for Isolation and Detection of EVs: Advantages Clarified from Comparison with Conventional Methods

Presenter:

Sadamu Ozaki, FUJIFILM Wako Pure Chemical Corporation

Abstract:

FUJIFILM Wako introduces a novel affinity purification method using Tim4 for isolating and detecting extracellular vesicles (EVs). Our new method enables easy isolation of high purity and high yield EVs from cell culture medium and body fluids, without ultracentrifugation. In this seminar, we will introduce the main features of PS Affinity.


A light lunch will be served.

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Date and Time

Location

FUJIFILM Diosynth Cambridge Collaboration Center (C3)

One Kendall Square, Binney St

Building 400

Cambridge, MA 02139

View Map

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