KGI & BPAT Presents: Mammalian Cell Culture Intensive Training ~ July 12 -...

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Keck Graduate Institute

535 Watson Drive

Claremont, CA 91711

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Mammalian Cell Culture

Bioprocessing Professional Advancement & Training (BPAT)
Industry Training Program

Date: July 12 - 14, 2017
Location: Amgen Bioprocessing Center, Keck Graduate Institute

Who should apply?

KGI has designed this MCC course for a broad range of industry skills and experiences. The class will provide practical operations training for industry professionals with little/no lab experience as well as for those with considerable bioprocessing background.

Course program synopsis:

Advances in mammalian cell technology over the past three decades, combined with major developments in therapeutic proteins and antibodies, have made the recombinant Chinese Hamster Ovary (CHO) cells the expression of choice in biomanufacturing.

In traditional mechanically stirred bioreactors, production levels have increased 10,000-fold during the same period and product protein titers are now routinely measured in multigrams per liter. These achievements are leading to significant cell culture bioprocess sophistication, and, along with emerging single use technologies, are making the future flexible bioprocess manufacturing a distinct possibility.

This three day intensive course is designed for professional scientists and engineers who wish to enhance their basic knowledge and training in upstream cell culture operations.

The primary goal of the course is to update the participants’ background in mammalian cell operations, from media selection and optimization to bioreactor design and operation and scale-up/scale-down.

Course overview

Scientific, engineering, and practical industrial aspects will be presented in a series of lectures, seminars, and workshops complemented with case studies and short laboratory demonstrations in KGI’s Amgen Bioprocessing Center. Course attendees will gain first-hand experience in principles of mammalian cell culture technologies, process development, scale up and scale down, control and measurement of dissolved oxygen, pH and tempereature, setting and running experiments, and analyzing data from high-density, fed-batch cultures in lab scale bioreactors.

Quotes from previous course attendees:

"The BPAT Courses have been an exceptional tool for learning the in-depth theory behind the bioprocessing techniques that we work with every day." - Victor Constantinescu, Manager, Purification, SHIRE

"Besides delivering targeted subjects, one of the best parts about the KGI BPAT workshop (Viral Safety in Biomanufacturing) was the high ratio of instructors to students - creating a comfortable intimate setting that fostered interactive learning. This approach is unique to anything I've experienced to date, and as a result, I was able to conveniently build relationships with SMEs in the field," - Sheldon Mink, PhD, Sr. Manager, QC Virology, Regenron, Inc.

"Every time I visit KGI for their BPAT courses, I'm amazed by the engagement from the instructors, especially Dr. Parviz Shamlou...KGI is a well-respected school within the biopharma industry and their campus is modest for what they have to offer." - Christine Bui, Sr. Manager, Manufacturing, Portola Pharmaceuticals, Inc.


This annual three-day intensive training course will follow the highly successful format established in 2014, integrating lectures and projects in a classroom setting with access to lab-scale work based on participant's experience and training needs.

In general, lectures and projects will occur in the morning, with case studies, lab demonstrations, and advanced project discussions in the afternoon; these will be in a break-out session based on participants’ experience.

This format maximizes content appropriateness for each participant, offers the opportunity to apply lecture material to a lab or project/computational experiment, and provides ample time for student-instructor interaction.

Lecture topics

  • Cell culture bioreactor design and scale-up and scale-down models
  • Impacts of agitation and aeration on cells
  • Batch vs. fed-batch vs. perfusion bioreactor
  • pH measuerment and control
  • Dissolved oxygen measurement and control
  • Volumetric Oxygen transfer rate and its impact on cells growth
  • Impact of shear on cell growths
  • Design and operation of single use bioreactors
  • Media selection and optimization including impact of trace metals
  • Integration of cell culture processes with downstream product recovery and purification
  • Key issues and tools used in industrial cell culture operations, including economies of scale, control charts, and process flow diagrams
  • Measurement of cell growth, death, and primary metabolism
  • Performance of mammalian cell culture operations under current Good Manufacturing Practices (cGMPs)
  • Cleaning, sterilization, set-up, and operation of a laboratory-scale bioreactor, for growth of a high-density, fed-batch suspension culture (laboratory session)
  • Measurement, tracking, and control of culture pH, oxygen, temperature, and other environmental parameters (laboratory session)
  • Cell culture Process Analytical Technology (PAT)

Lead instructors

  • Parviz Shamlou –KGI, Lilly, UCL
  • Ashraf Amanullah - Merck, genentech, Gilead, aTyr Pharmaceutical
  • Tom Fletcher, Irvine Scientific
  • Jon Wannlund - BD Biosciences (TBC)
  • Robert Garrahy - Broadley James
  • Lab demonstration sessions led by KiriLynn Svay - KGI


The MCC course will be held at Keck Graduate Institute School of Applied Life Sciences, 535 Watson Drive, Claremont CA 91711.

KGI is approximately 12 miles from the Ontario, California airport


Deadline for the Early Bird Fee of $2,500.00 is the end of day June 9th, 2017. Beginning June 10th, the full cost of the course is $3,000.00. Included within the fee are all lab equipment and supplies, handouts and materials, instruction, daily breakfast and lunch will be included, as well as two evening dinners. Accommodations and travel expenses are the participant’s responsibility. Below are several nearby hotel suggestions conveniently located near KGI.

*Special Discount Rate for KGI Alumni! For further information and pricing, please contact:

Christa Oudshoorn-Hobo
KGI Alumni Engagement Manager
Office: 909-607-8592


Cancellation Requests for refunds due to cancellation for any reason must be made in writing to aa.events@kgi.edu

Requests for refunds received by us between June 15 - July 1, 2017 will be made, minus a $500.00 processing fee.

NO refunds will be given for any reason starting July 1, 2017


-Hotel Casa 425: http://www.casa425.com/
*This hotel is within walking distance to KGI, located around the corner from the campus.

Doubletree by Hilton Hotel – Claremont:
http://doubletree3.hilton.com/en/hotels/california/doubletree-by-hilton-hotel-claremont- ONTCLDT/index.html
*This hotel is located less than 2 miles from the KGI Campus.

Sheraton Fairplex Hotel & Conference Center: http://www.sheratonfairplex.com/
*This hotel is located less than 4 miles from the KGI Campus – Approx. a 7 min. drive.

International Airport:

The closest airport to Keck Graduate Institute is the Ontario International Airport:http://www.lawa.org/welcomeONT.aspx

Other close-by airports include:

John Wayne/Orange County Airport: http://www.ocair.com/

The furthest from KGI would be the Los Angeles International Airport:


For further information contact:

KiriLynn “Lynn” Svay - BPAT Program Director
Office: 909-607-0122
Cell: 714-482-5327


Keck Graduate Institute


Please Advise of any special dietary restrictions when registering.

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Keck Graduate Institute

535 Watson Drive

Claremont, CA 91711

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