This short course, offered by the Donald Danforth Plant Science Center’s Integrated Microscopy Facility, provides a solid background on the use of light microscopy, especially confocal microscopy, as a tool to study cell and molecular biology. The course emphasizes imaging of plant tissues but its scope is not limited to this. The course consists of morning lectures that provide theoretical background in various aspects of light microscopy, and afternoon lab sessions that give the student hands-on experience in operating a confocal microscope, the Integrated Microscopy Facility’s Leica SP-8 confocal microscope. Students will not need to attend afternoons except for their scheduled hands-on training (two hour) session(s). The course is designed for beginning and intermediate users of confocal microscopy, and for those wanting a refresher on confocal and light microscopy.
For syllabus and schedule information, check for updates at www.danforthcenter.org
When & Where
The facility director, R. Howard Berg, has considerable experience in the study of plant cell structure. He serves as a resource for users in designing and implementing their imaging experiments. Confocal microscopy requires a solid background in understanding how to optimally acquire image data and prepare it for publication. To that end, Howard conducts comprehensive multi-day workshops in confocal microscopy, offered in January and July. These have been popular, with an average of 12 students per workshop to date. A series of workshops in transmission electron microscopy techniques are now being designed and will be offered in the future.