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Introduction to Multiphoton Microscopy and Fluorescence Lifetime Microscopy

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Ontario Institute for Cancer Research

661 University Avenue

West Tower, Suite 510

Toronto, ON M5G0A3

Canada

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Seminar Series: Cell Imaging

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A confocal microscope based on visible light can leave many details invisible. Often the image you need is hidden deep within tissue, which not only scatters light but is damaged by it too. The excellent optical design of the Leica TCS SP8 DIVE/FALCON system combines use of long wavelengths of fully integrated infrared excitation lasers, the high efficiency of HyD detectors, and an infrared-optimized optical transmission. These components allow deeper penetration into the tissue with super-sensitivity to uncover the finest details of cellular and subcellular processes. This system is the best for thick organ sections, intact organs or entire live model organisms.

Learning objectives:

• Why and when to use multiphoton microscopy

• Principles of multiphoton microscopy

• Applications

Presenter:

Lianne Dale, Confocal Application Scientist, Leica

Additional Information:

Vanya Peltekova, PhD, BioLab Lead, OICR:

vanya.peltekova@oicr.on.ca

On Demand may become available for this seminar.

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Ontario Institute for Cancer Research

661 University Avenue

West Tower, Suite 510

Toronto, ON M5G0A3

Canada

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