Introduction to Flow Cytometry Course- May 2021.

Designed for those new to flow cytometry this popular interactive group training session will for the first time be an online class. Experienced Flow Cytometrists David Onion and Adriana Flores-Langarica will lead this comprehensive Introduction to Flow Cytometry, which includes live practical demonstrations.

This session is for staff and students of the Midladns Innovation Universities.

Programme

13:00-14:00 Session 1

• What is flow cytometry and how does it work?

• Applications of flow cytometry in biomedical research.

• What is cell sorting and how does it work?

• What is Imaging Cytometry and how does it work?

• Information / further reading / useful websites / analysis software.

14:00-14:15 Break

14:15-15:15 Session 2

• Reagents for flow cytometry: antibodies, fluorophores and more.

• Basic design of flow cytometry experiments.

• Panel design, colours that overlap and compensation.

• Setting up a flow cytometer.

15:15-15:30 Break

15:30-16:30 Live Flow Cytometry Demonstrations

• Creating a protocol.

• Setting voltages.

• Setting compensation.

For further information

e-mail David Onion or Adriana Flores-Langarica or your local Midlands Innovation Flow Group Member:

Aston University Jill Johnson, Cranfield University Francis Hassard, University of Birmingham  Adriana Flores-Langarica and  Andrea Mitchell, DMU Naomi Martin, University of Keele Alan Richardson, University of Leicester David Cousins, Loughborough University Christof Leicht, University of Nottingham David Onion and Lucy Fairclough, University of Warwick Dan Hebenstreit.

 https://miflowgroup.weebly.com/