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Improving your Exome sequencing [SV4-0418]

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European Research Institute for the Biology of Ageing, University Medical Center Groningen, ERIBA building (3226) - 3e floor - Meeting room 03.14

Antonius Deusinglaan, 1

9713 AV Groningen

Netherlands

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Improving exome sequencing, targeted sequencing, and low frequency variant detection with better coverage uniformity, higher on-target rates, and unique molecular identifiers

How better uniformity and higher on-target rates can improve your Exome NGS project.

* High level introduction of NGS library preparation and target enrichment with IDT NGS solutions

* Main challenges of the NGS target enrichment in the current market: uniformity, on-target rate and the capture of high GC content region

* IDT developed xGen Exome Research Panel as a great solution of those market challenges, proved by a comparative benchmark study run by an independent party.

* Statement of IDT & Illumina partnership of Exome sequencing solution.

Accurate detection of low frequency genetic variants with Unique Molecular Identifier application

*There is a growing need for accurate detection of low frequency variant.

*Main challenges of the low frequency variant detection: low amount input DNA, bad quality DNA (FFPE), PCR errors in NGS library amplification steps.

*IDT developed UMI (Unique molecular Identifier) adapter, which could be used to track all PCR duplicates of library DNA fragments and eliminate those PCR errors. As a consequence, accurate low frequency genetic variants detection could be achieved.

Date and Time

Location

European Research Institute for the Biology of Ageing, University Medical Center Groningen, ERIBA building (3226) - 3e floor - Meeting room 03.14

Antonius Deusinglaan, 1

9713 AV Groningen

Netherlands

View Map

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