Free

Hands-on Electrophoresis and PCR Workshop

Event Information

Share this event

Date and Time

Location

Location

Lok Sin Tong Yu Kan Hing Secondary School

View Map

Friends Who Are Going
Event description

Description

Join MiniOne® Systems for a hands-on DNA electrophoresis and PCR workshop,

Graciously hosted by Mr. Man & the Science Team.

Workshop is limited to 25 attendees, so RSVP now!


Workshop Schedule:

Sign in: 9:30 – 9:45 am

Introduction: 9:45 – 10:00 am

Workshop: 10:00 – 12:00 noon

Q & A: 12:00 – 12:30 pm


Concepts covered in workshop:


The basics of using an adjustable micropipette and electrophoresis:

  • Pipetting microliters of samples

  • Casting an agarose gel

  • Loading microliters of sample into an agarose gel

  • Running and seeing DNA bands move in real time

  • Capturing the gel image on a mobile device


The basics of doing a PCR:

  • Pipetting and transferring microliters of samples

  • Setting up a PCR reaction

  • Programming a PCR protocol

  • Running a PCR reaction

  • Analyzing the results and compare to prediction

Biotech and life science concepts:

  • Principles in separation of macromolecules like DNA

  • Concept behind paternity testing - mammals are diploid!

  • What is PCR and how does it work - intellectually

  • Real world understanding of how PCR works by calculating and deriving the size of DNA fragments generated using a portion of the Lambda phage genome and three pairs of primer set.
Share with friends

Date and Time

Location

Lok Sin Tong Yu Kan Hing Secondary School

View Map

Save This Event

Event Saved