Hands-on Electrophoresis and PCR Workshop

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Lok Sin Tong Yu Kan Hing Secondary School

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Join MiniOne® Systems for a hands-on DNA electrophoresis and PCR workshop,

Graciously hosted by Mr. Man & the Science Team.

Workshop is limited to 25 attendees, so RSVP now!

Workshop Schedule:

Sign in: 9:30 – 9:45 am

Introduction: 9:45 – 10:00 am

Workshop: 10:00 – 12:00 noon

Q & A: 12:00 – 12:30 pm

Concepts covered in workshop:

The basics of using an adjustable micropipette and electrophoresis:

  • Pipetting microliters of samples

  • Casting an agarose gel

  • Loading microliters of sample into an agarose gel

  • Running and seeing DNA bands move in real time

  • Capturing the gel image on a mobile device

The basics of doing a PCR:

  • Pipetting and transferring microliters of samples

  • Setting up a PCR reaction

  • Programming a PCR protocol

  • Running a PCR reaction

  • Analyzing the results and compare to prediction

Biotech and life science concepts:

  • Principles in separation of macromolecules like DNA

  • Concept behind paternity testing - mammals are diploid!

  • What is PCR and how does it work - intellectually

  • Real world understanding of how PCR works by calculating and deriving the size of DNA fragments generated using a portion of the Lambda phage genome and three pairs of primer set.
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Lok Sin Tong Yu Kan Hing Secondary School

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