$25

Glow Germ and Bacterial Transformation (Home School)

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The Citizen Science Lab

1435 Bedford Ave

Suite 132A

Pittsburgh, PA 15219

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Students get a chance to create their own glowing bacterial art. Students use non-pathogenic E. coli that expresses fluorescent proteins to draw their image. Students can choose between green, yellow, blue, red, and orange fluorescent bacteria or use a combination of all five. The plates are incubated overnight and the following day students get to view their glowing bacteria art under a black light.

While we usually think of genetic engineering as the stuff of science fiction, or at least cutting-edge research, in simple organisms it's surprisingly easy. In this module, students will create their own glowing bacteria through the true gene transfer of a plasmid coding for a fluorescent protein. In the process, they will learn about bacterial culturing, ascetic technique, and horizontal gene transfer.

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The Citizen Science Lab

1435 Bedford Ave

Suite 132A

Pittsburgh, PA 15219

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