$200 – $400

Genome Editing with CRISPR-Cas9

Event Information

Share this event

Date and Time

Location

Location

Genspace

132 32nd Street

Suite 108

Brooklyn, NY 11232

View Map

Refund Policy

Refund Policy

Refunds up to 7 days before event

Eventbrite's fee is nonrefundable.

Event description

Description

Four-part course:

Friday, November 15th, 6-9pm
Saturday, November 16th, 2-6pm
Sunday, November 17th, 2-6pm
Sunday, November 24th, 2-6pm

CRISPR/Cas9 is one of the most transformative recent developments in biotechnology, radically simplifying the process of editing genomes and increasing its accessibility. Want to learn how to do hands-on genome editing? This is an intensive laboratory class with limited space. Class size is limited to ten students.

In this class you will:

  • Learn how to culture and work with Saccharomyces cerevisciae (brewers yeast)
  • Assemble a plasmid encoding a CRISPR/Cas9-mediated gene deletion
  • Generate a repair template encoding a fluorescent protein that will be inserted into the yeast genome
  • Use the constructs you produce to transform yeast and confirm the results by DNA sequence analysis

Prerequisites:

  • You must be familiar with molecular biology lab techniques such as pipetting, gel electrophoresis, use of restriction enzymes and PCR (Genspace Biohacker Boot Camp or equivalent lab experience)


Dr. Neta Agmon received her Bachelors of Science in Biology from Tel Aviv University in Israel. She received her Master of Science in Genetics from Tel Aviv University, studying the mechanism of formation of extrachromosomal circular DNA and its replication by rolling circle. She received her PhD from the department of Molecular Microbiology and Biotechnology in Tel Aviv University studying the choice of mechanism for the repair of a Double Strand break in the budding yeast Saccharomyces cerevisiae. In 2012 she moved to the US to work on building multigene pathways in yeast cells at Johns Hopkins University School of Medicine in the lab of Dr. Jef Boeke. The lab relocated to New York University Langone School of Medicine at 2014. Her research interests are genetics and synthetic biology and using budding yeast as a model system for human diseases.



Dr. Paolo Mita is a post-doctoral fellow in Dr. Jef Boeke's laboratory since 2012. He obtained the laurea in Biological Sciences with a trend in molecular biology at the University of Lecce (Italy) and subsequently worked at the Italian Center of Research (CNR) and Universita’ Federico II in Naples (Italy) under the supervision of Dr. Scarfi’ and Dr. Abrescia, focusing on bio-electromagnetism, apoptosis, and atherosclerosis. In 2006, Dr. Mita moved to New York and obtained a joint PhD at NYU medical school and Universita’ di Milano in 2012, working on androgen receptor transcriptional regulation, under the supervision of Dr. Susan Logan. In Dr. Boeke's laboratory, Dr. Mita is exploring the interactions of LINE-1 retrotransposon with human host cells trying to identify key cellular regulators of LINE-1 activity.

Share with friends

Date and Time

Location

Genspace

132 32nd Street

Suite 108

Brooklyn, NY 11232

View Map

Refund Policy

Refunds up to 7 days before event

Eventbrite's fee is nonrefundable.

Save This Event

Event Saved