$400

Genome Editing with CRISPR-Cas9

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Genspace

140 32nd Street

Suite 108

Brooklyn, NY 11232

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Class in four sessions:

December 4, 5, 6 and 13

6:00 PM - 10:00 PM

CRISPR/Cas9 is one of the most transformative recent developments in biotechnology, radically simplifying the process of editing genomes and increasing its accessibility. Want to learn how to do hands-on genome editing? This is an intensive laboratory class with limited space. Class size is limited to ten students.

In this class you will:

  • Learn how to culture and work with Saccharomyces cerevisciae(brewers yeast)

  • Assemble a plasmid encoding a CRISPR/Cas9-mediated gene deletion

  • Generate a repair template encoding a fluorescent protein that will be inserted into the yeast genome

  • Use the constructs you produce to transform yeast and confirm the results by DNA sequence analysis

Prerequisites:

  • You must be familiar with molecular biology lab techniques such as pipetting, gel electrophoresis, use of restriction enzymes and PCR (Genspace Biotech Crash Course, Biohacker Boot Camp or equivalent lab experience)

Will Shindel


Will Shindel is the Laboratory Manager at Genspace and also provides scientific mentoring to our members. He studied Biology at Arcadia University, where he investigated the role of insulator elements in genome organization. After graduating, he spent several years working in the pharmaceutical industry. It was during this time that he discovered DIY biology and first came to Genspace. In addition to being Laboratory Manager, Will also teaches the course Genome Editing with CRISPR Cas-9 and is working on creating a spicy tomato by engineering in the capsaicin pathway genes.

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Genspace

140 32nd Street

Suite 108

Brooklyn, NY 11232

View Map

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