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Flow Cytometry Bootcamp Cursos Edition - ICMAN-CSIC Spain

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Instituto de Ciencias Marinas de Andalucia (ICMAN)

Campus Universitario Río San Pedro s/n

Rio San Pedro 11519

Pto. Real - Cádiz · Spain

Spain

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Description

Educational Objectives

Education Objectives: This two day course will cover the fundamentals of flow cytometry to provide the critical concepts for the novice and intermediate users. This course focuses on the core concepts of flow cytometry – the ‘essentials’ that every scientist using flow should know. At the end of the course, students will have a better understanding of the complexities of flow cytometry experimental design, optimization and analysis.

Target Audience

Users with basic flow cytometry knowledge seeking to understand the essential concepts in flow cytometry and a desire to move into higher-end assays (from 4 color to 8+ color experiments). Learning these principles will ensure your flow cytometry experiments will yield results – ultimately saving you time and money in the long run.

Course Outline and Schedule

Please note all times are approximate, based on the degree of audience participation.


Instructor: Alfonso Blanco-Fernandez, Director of the Flow Cytometry Facility UCD-Conway Institute, Dublin, Ireland.


Day 1

09:00-09:30 Course Introduction and Activity 1 – Design the experiment

09:30-10:15 Lecture 1 – Reagents (Fluorescence, Fluorochromes and Reagents)

10:15-10:45 Activity 2 – Fluoresce and Spectral Viewers

10:45-11:00 Coffee Break and questions

11:00-11:45 Lecture 2 – Hardware (Flow Cytometry Hardware)

11:45-12:30 Lecture 3 – Compensation Principles and Practices

12:30-13:30 Lunch and Sponsor talk

13:30-14:00 Activity 3 – Compensation Practice

14:00-14:45 Lecture 4 – Understanding Flow Cytometry Data

14:45-15:30 Lecture 5 – Controls for Interpreting Flow Cytometry Data

15:30-15:45 Coffee break and Questions

15:45-16:30 Lecture 6 – Theory of Panel Design

16:30-17:00 Questions and Day 1 wrap-up


Day 2

09:00-10:15 Activity 4 – Panel Design

10:15-10:30 Coffee Break and Questions

10:30-11:30 Lecture 7 – Statistics in Flow Cytometry

11:30-12:30 Activity 5 – Principles of Data Analysis

12:30-13:30 Lunch and Sponsor talk

13:30-14:15 Lecture 8 – Rare Event Analysis

14:15-15:15 Activity 6 – Flow Cytometry Case Study

15:15-15:30 Coffee Break and Questions

15:30-15:45 Review of Activity 6

15:45-16:30 Lecture 9 – Troubleshooting Flow Cytometry Experiments

16:30-17:00 Questions and Course wrap-up


Note: a computer with analytical software is helpful but not critical.

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Date and Time

Location

Instituto de Ciencias Marinas de Andalucia (ICMAN)

Campus Universitario Río San Pedro s/n

Rio San Pedro 11519

Pto. Real - Cádiz · Spain

Spain

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