$325 – $375

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The Ohio State University Medical Center

Columbus, Ohio 43210

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Educational Objectives

Education Objectives: This two-day course will start by covering the fundamental areas critical for successful flow cytometry: Reagents, Hardware, Controls, Compensation, and Data Analysis. The second day will build upon these concepts with a focus on practical application of flow cytometry including: Polychromatic Panel Design, Statistics, Cell Sorting Principles and Troubleshooting. At the end of the course, attendee’s will have firm understanding of the principles and how these apply to developing, executing, analyzing and troubleshooting flow cytometry experiments

Target Audience

Users with basic flow cytometry knowledge seeking to understand the essential concepts in flow cytometry and a desire to move into higher-end assays (from 4 color to 8+ color experiments). Learning these principles will ensure your flow cytometry experiments will yield results – ultimately saving you time and money in the long run.

Day One Agenda

09:00-09:30 Course Introduction and Activity 1 – Design the Experiment

09:30-10:15 Lecture 1 – Software (Reagents, Fluorescence and Fluorochromes)

10:15-10:30 Introduction to Spectral Viewers

10:30-10:45 Coffee Break and questions

10:45-11:30 Lecture 2 – Hardware (Fluidics, Optics and Electronics)

11:30-12:15 Lecture 3 – Compensation Principles and Best Practices

12:15-13:15 Lunch and Sponsor Talk

13:15-14:00 Activity 2 – Compensation practice (w/data)

14:00-14:45 Lecture 4 – Controls in Flow Cytometry

14:45-15:30 Lecture 5 – Understanding Flow Cytometry Data

15:30-15:45 Coffee Break and questions

15:30-16:30 Lecture 6 – Optimization and Standardization in Flow Cytometry

16:30-17:00 Activity 3 – Test yourself

Day Two Agenda - Complex Data

09:00-09:15 Questions from Day 1

09:15-10:00 Lecture 7 – Theory of Polychromatic Panel Design

10:00-10:30 Lecture 8 – Automation in Panel Design

10:30-10:45 Coffee Break and Questions

10:45-12:00 Activity 4– Panel design

12:00-13:00 Lunch and Sponsor Talk

13:00-13:30 Review of Panel Design

13:30-14:30 Lecture 9 – Statistics in Flow Cytometry

14:30-15:30 Lecture 10 – Practical Cell Sorting

15:30-15:45 Coffee Break and questions

15:45-16:30 Lecture 11 – Troubleshooting your data

16:30-17:00 Activity 6 – Final Quiz

Note: a computer with analytical software is helpful but not critical.

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The Ohio State University Medical Center

Columbus, Ohio 43210

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