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ExCyte's Flow Cytometry Compensation Course - Beyond The Basics

Carol Oxford; ExCyte Co-Founder and Former Core Director UC Davis (25 Years)

Thursday, 29 August 2013 from 13:00 to 14:00 (EDT)

ExCyte's Flow Cytometry Compensation Course - Beyond...

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Ticket Type Sales End Price Fee Quantity
ExCyte's Flow Cytometry Compensation Ended US$15.00 US$1.82

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Event Details

Did you know that compensation is not about choosing the correct antibody? If you want to publish your flow data and get funded based on your flow data, you will have to learn the physics of fluorescence.

Fluorescent flow cytometry is a powerful tool for understanding and characterizing complex cellular phenotypes. With the first paper in 17 color flow published in 2004, investigators and manufacturers rushed to use higher end instruments that could measure more and more fluorochromes. Quickly, the fluorescent spectrum became a very crowded space. The problem was and is the physics of fluorescence.

Too many promising research projects are stymied by their antibody panel's lack of sensitivity. Compensation is a critical parameter to get correct for any flow cytometry expriment that involves more than one color. Failure to compensate correctly leads to artifacts, false positives, incorrect conclusions, and negative reviews.

During this lecture, the best practices for compensating any type of flow cytometry or cell sorting experiment will be discussed. In particular, the three rules of proper compensation will be outlined, as well the consequences of improper compensation. By the end of the lecture, every attendee will understand how to recognize good and bad compensation and how to perform proper compensation quickly and easily.

Purchasing an Eventbrite ticket will give you exclusive access to the this seminar, which is hosted by Fuze Box (an online seminar platform), and will allow you to interact directly with the workshop instructors by asking questions using Fuze Boxe's Q&A tool. An hour before the seminar, you will receive an access link via email that will allow you to join the seminar on Fuze Box, a seminar hosting server.

Do you have questions about ExCyte's Flow Cytometry Compensation Course - Beyond The Basics? Contact Carol Oxford; ExCyte Co-Founder and Former Core Director UC Davis (25 Years)

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Organiser

Carol Oxford; ExCyte Co-Founder and Former Core Director UC Davis (25 Years)

Carol Oxford is a Staff Scientist in R&D at Bio-Rad Laboratories (Hercules, CA), where her primary responsibility is the new S3 cell sorter. Carol develops new applications, and trains the sales and technical support staff in the US and around the world. I started this position in October of 2012.

Previously, Carol was the Director of the Shared Resource Lab at UC Davis for 25 years. Carol ha worked with the UCD Cancer Center to establish the first UCD Shared Resource Laboratory for Flow Cytometry and Cell Sorting in 1995. She then became Technical Director in 1997. Carol's efforts were instrumental in developing a facility that went from having only a Becton Dickinson cell analyzer to a facility having two state of the art cell sorters, six flow analyzers, and a laser scanning cytometer. Carol has attended the Los Alamos National Flow Cytometry Courses, as well as training courses provided for specific instruments by Becton Dickinson, Beckman Coulter, and Cytomation.

Carol has attended 11 different meetings of the International Society for Analytical Cytology. As the SRL Director, she oversaw the specification and purchasing of new equipment, and consulted with investigators about the design of experiments, optimization of protocols, and analysis of data.

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