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Advanced solutions for CRISPR & qPCR applications [VR11272019]

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Aix Marseille Université

Bâtiment TPR2-bloc5 (salle de réunion)

163, Avenue de Luminy

13288 Marseille

France

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Description

Advanced solutions for CRISPR & qPCR applications

Join us to :

- learn more about CRISPR genome editing & qPCR/genotyping solutions

- learn more about IDT's latest developments in order to drive performance improvement

- have the opportunity to discuss with IDT's specialists and other users about your specific applications in those areas

Detailed program:

9:00 – 10:30: Optimizing CRISPR strategy to achieve potent and precise genome editing

Speaker: Lai Chan – Field Application Manager Functional genomics

During this presentation you will learn about :

  • Optimized strategies for CRISPR-Cas9 mediated genome editing, with emphasis on methods commonly used for delivery of guides RNAs and Cas9 as a ribonucleoprotein complex
  • The risk of off-target effects (OTEs): how to find and measure the frequency of OTEs including discussion of bioinformatic screening for OTEs and methods for unbiased CRISPR OTEs detection
  • Recent developments in CRISPR research, including a novel mutant Cas9 protein that has been evolved to reduce off-target gene editing while maintaining ontarget potency
  • And it will be followed by an open discussion about your specific project in term of design, protocols, difficulties, ...)


10:45 – 12:15: Development of qPCR assays - detection and quantification of CRISPR editing

Speaker: Richelle Spanjers – Field Application Manager Gene Expression & Genotyping

During this presentation you will learn about :

  • Steps to a successful PCR, qPCR & dPCR experiment including:
    • T7E1 screening
    • Quantification of Gene Knock out
    • Quantification of Gene Knock in
    • Detection of SNP introduction
  • We will also discuss assay design criteria
    • for gene expression (qPCR & dPCR)
    • for genotyping (LNA, MGB & rhAmp SNP)
  • And it will be followed by an open discussion about your specific project in term of design, protocols, difficulties, ...)
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Date and Time

Location

Aix Marseille Université

Bâtiment TPR2-bloc5 (salle de réunion)

163, Avenue de Luminy

13288 Marseille

France

View Map

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