€287.85

Advanced Panel Design in Amsterdam

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National Cancer Institute

Plesmanlaan 121

1066CX Amsterdam

Netherlands

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18-Color Flow Cytometry

NKI Amsterdam Edition



Educational Objectives: This one-day seminar will go through the steps necessary to develop and implement 10+ color flow cytometry panels. Beginning with the design of a panel, we move through the critical steps of instrument optimization, titration, and voltage determination using real-world data. We will work through the standardization of the signals over time, optimizing data analysis while emphasizing the use of critical controls. At the end of the class, attendees will have the necessary skills to build new and improve existing polychromatic flow cytometry panels.

Target Audience: Researchers with more than 6 months experience and who are seeking to expand their current flow cytometry panels into 10+ colors.



Sponsored by:

BioLegend


Course Outline

Iyadh Douagi, Instructor

09:00-09:15 Instructor Introduction

09:15-10:00 Panel 401 – Hardware Optimization

10:00-10:30 Panel 402 – Brightness

10:30-10:45 Coffee Break

10:45-11:30 Panel 403 – Theory of Panel Design

11:30-12:00 Panel 404 - Automation in Panel Design

12:00-13:00 Lunch

13:00-14:00 Group Activity – Panel Design

14:00-14:30 Interactive Panel Design II – Review and Discussion

14:30-15:30 Panel 405 – Principles of Panel Optimization and Standardization

15:30-15:45 Coffee Break

15:45-16:45 Panel 601 – Statistics in Flow Cytometry

16:45-17:00 Final Questions

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Date and Time

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National Cancer Institute

Plesmanlaan 121

1066CX Amsterdam

Netherlands

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