Advanced Multicolor Antibody Panel Design Webinar (2017)
Learn How To Create The Right Multicolor Flow Cytometry Antibody Panel Every Time, including:
- How to utilize new fluorochromes such as Brilliant Violet dyes to create better antibody panels.
- 3 most common panel design mistakes most researchers make.
- The 10 steps required to create a correct antibody panel for any flow cytometer.
- Why instrument optimization is critical for successful panel design and the resources necessary to optimize your instrument.
- How viability dyes and dump channels can improve the sensitivity and effectiveness of your antibody panel.
- What the brightness of a fluorochrome means and how to measure it.
- What questions you need to consider concerning the antigens you're targeting with your antibody panel.
- How to completely automate the antibody panel design process using the variety of online tools that are now available--this will cut your work time in HALF!
- The exact sequence for optimizing a polychromatic antibody panel, including one unique "trick" that very few researchers know about.
- The top resources available to you for when you get stuck--we've researched and collated all the best tool and sites so you don't have to.
- How to use OMIPs and how to share your antibody panels with other researchers for quick feedback.
- How to properly submit your antibody panels to reviewers so they don't reject them (this happens more than you think).
*This webinar will be recorded and sent to you if you join and can't make it to the live event.
Polychromatic flow cytometry allows for the identification and characterization of complex phenotypes using fluorescently labeled antibodies and fluorescent cell probes. The process of developing a panel requires a balance between choosing the best fluorochromes to make sensitive measurements, while minimizing the loss of sensitivity in a given channel due to the fluorescent spillover resulting from the other fluorochromes in the panel.
While this sounds simple in concept, the practice of building a panel is anything but. One must have a sound understanding of the instrument to be used, including a detailed characterization of the system--where it can make a sensitive measurement, what channels are best suited for dump channels etc.
Brightness of a given fluorochrome is another important consideration and piece of information that will aid in the development of a panel. Of course, when all else fails, one can turn to the OMIP for help and guidance. This webinar will provide the theoretical foundation for the considerations necessary to develop a polychromatic flow cytometry panel.
Purchasing an Eventbrite ticket will give you exclusive access to the this seminar, which is hosted by Fuze Box, and will allow you to interact directly with the workshop instructors by asking questions using Fuze Box's Q&A tool. After the seminar, you will also be given a link to download a video of the course for free to watch later.
An hour before the seminar (12PM EST), you will receive an access link via email that will allow you to join the seminar on Fuze Box, a seminar hosting server. The seminar starts at 1PM EST. ***Your ticket is also your receipt.