6eDTEW 6th Annual Environmental DNA Technical Exchange Workshop

The 6th annual Environmental DNA Technical Exchange Workshop (6eDTEW) will be a virtual workshop hosted and coordinated by the Government eDNA Working Group (GEDWG). GEDWG is a no-cost consortium that focuses on bringing together scientists, natural resources managers, and other stakeholders interested in eDNA and related fields, for the purposes of sharing technical expertise and experience. Participants from outside federal, state, provincial, municipal, and other government agencies are welcome to join. For more information on GEDWG, please contact Richard Lance (richard.f.lance@erdc.dren.mil) or Katy Klymus (kklymus@usgs.gov). Abstract submission: https://forms.gle/GXnDBjooasHiAeLKA

6eDTEW

6th Annual eDNA Technical Exchange Workshop

All times are in Eastern Time (ET) aka New York

Tuesday, January 24, 2023

12:00 PM ET (UTC -5)

Welcome and Logistics (Richard Lance)

12:10 –16:45

Session 1: eDNA basics and standards

Chair: Richard Lance Chat Monitor: Katie Fillingham

12:10 –13:00

13:00 –13:50

Session 1 Plenary 1, David Lodge/ Accelerating the use of eDNA in policy and management (Cornell University)

Session 1 Plenary 2, Jennifer Tank/ Predicting eDNA transport and degradation in flowing waters (University of Notre Dame)

13:50 – 14:10

Break

14:10

John Kronenberger/ Predicting qPCR cross-amplification with high accuracy (USFS National Genomics Center for Wildlife and Fish Conservation)

14:30

Anna Mangan/ Validation of a nutria (Myocastor coypus) environmental DNA assay highlights considerations for sampling methodology (USDA National Wildlife Research Center)

14:50

Richard Lance/ Elusive waders: Developing, Testing, and Validating eDNA Assays for Two Imperiled Rails (Environmental Laboratory, US Army Engineer Research & Development Center)

15:10 –15:25

Break

15:25

Matthew Yates/ A general framework to unify the estimation of numerical abundance and biomass from quantitative eDNA data (University of Windsor)

15:45

Mark Stoeckle/ 12S gene metabarcoding with DNA standard quantifies marine bony fish eDNA (The Rockefeller University)

16:05

Erik Pilgrim/ How many samples are enough? Trying to determine guidelines for fish eDNA sampling to capture full fish assemblages in lakes (U.S. EPA)

16:25

Ingrid Bunholi/ Environmental DNA and RNA in community ecology across the tree of life: a methodological review (Department of Marine Science, The University of Texas at Austin)

16:45 –16:50

Break

16:50 – 17:20

Poster Blitz 1

Chair: Sarah Brown Chat Monitor: Michael Powers

16:50

Kelsey Ruehling/ Comparison of enzymatic verses membrane DNA extraction methods for the sequencing of filtered freshwater samples (Wyoming Department of Environmental Quality)

16:55

Kasia Przybyla-Kelly/ Comparing round goby eDNA concentrations in water, sediment, and algae with abundance estimates by divers in four Great Lakes (USGS)

17:00

Joseph Craine/ Development of a parallelizable, low-cost acquisition and filtration system for oligotrophic water (Jonah Ventures)

17:05

Dannise Ruiz Ramos/ Quantification of environmental DNA shedding and degradation rates from freshwater mussels (USGS Columbia Environmental Research Center)

17:10– 17:20

Poster Blitz Question & Answer

17:20 –17:40

Open discussion Session (John Darling)

17:40

Adjourn

Wednesday, January 25, 2023

10:00 ET

(UTC -5)

Welcome and Logistics: (Stephen Spear)

10:10 –13:20

Session 2: eDNA surveillance and monitoring networks

Chair: Stephen Spear Chat Monitor: Katy Klymus

10:10 –11:00

Session 2 Plenary, Adam Sepulveda/ Research to operations for eDNA early detection of invasive species (USGS Northern Rocky Mountain Science Center)

11:00-11:10

Break

11:10

Teun Everts/ The value of droplet digital PCR in eDNA surveys and biological invasion management programs (Research Institute for Nature and Forest & KU Leuven, Belgium)

11:30

Tiina Laamanen & Veera Norros & Kristian Meissner/ Roadmap for implementing environmental DNA (eDNA) and other molecular monitoring methods in Finland (Finnish Environment Institute)

11:50

Jessica Rieder/ Metagenomics and metabarcoding experimental choices and their impact on microbial community characterization in freshwater recirculating aquaculture systems (University of Bern, Switzerland)

12:10 –12:20

Break

12:20

Marion Chevrinais/ Targeted approaches of environmental DNA detections for aquatic invasive species in the St. Lawrence System, Canada (Fisheries and Oceans Canada)

12:40

Nicholas Schulte/ Environmental DNA reveals diversity and structure of freshwater algal assemblages across the conterminous United States (Jonah Ventures)

13:00

Teresita Porter/ STREAM: a community-based metabarcoding approach for freshwater ecosystem health assessment (Centre for Biodiversity Genomics, University of Guelph, Canada)

13:20 –14:10

LUNCH

14:10 –16:20

Session 3: Standards and Biodiversity

Chair: Kelly Goodwin Chat Monitor: Katy Klymus

14:10

Nicole Fahner/ Technical advances in target species detection using high-throughput eDNA sequencing (Centre for Environmental Genomics Applications, Canada)

14:30

Jason Ferrante/ NAS database (USGS)

14:50

Cathryn Abbott/ Canadian formal science advice on the use of targeted environmental DNA analysis, plus key considerations for future eDNA guidance products (Fisheries and Oceans Canada)

15:10 – 15:20

Break

15:20

Mark Johnson/ Environmental DNA metabarcoding from flowers reveals arthropod pollinators, plant pests, parasites, and potential predator-prey interactions while revealing more arthropod diversity than camera traps (University of Illinois Urbana-Champaign)

15:40

Amanda Boone/ Extraction of animal eDNA from dry and fresh flowers (USGS, Forest and Rangeland Ecosystem Science Center

16:00

Cathryn Abbott/ NAMERS: a Novel Applied eDNA Metabarcoding Reference Sequences portal to support eDNA implementation (Fisheries and Oceans Canada)

16:20 –16:30

Break

16:30 – 17:00

Poster Blitz 2

Chair: Adam Sepulveda Chat Monitor: Nastassia Patin

16:30

16:35

Camila MacLoughlin/ Eukaryotic eDNA metabarcoding improves biodiversity inventories by revealing underrepresented taxa in underwater visual surveys and historical records from the Gulf of California (Centro de Investigaciones Biologicas del Noroeste, La Paz, Mexico)

Caitlin Beaver/ Environmental DNA assessment of Burmese python occurrence north of their estimated established range (USGS, Wetland and Aquatic Research Center)

16:40

16:45

Carol Stepien/ Metabarcoding Identifications and Tracking of Invasive Species from eDNA and Bulk Samples: Implications for Management in Marine and Freshwater Aquatic Ecosystems (National Museum of Natural History, Smithsonian Institution)

Denise Lindsay/ Determining the Utility of eRNA as a Biomonitoring Tool

Poster Blitz Question and Answer (US Army Engineer Research and Development Center)

16:50 –17:00

Poster Blitz Question & Answer

17:00

Adjourn

Thursday, January 26, 2023

10:00 EDT

(UTC -5)

Welcome and logistics: (Maggie Hunter)

10:10-11:50

Session 4: Emerging Technologies and Automation

Chair: Mehrdad Hajibabaei Chat Monitor: Maggie Hunter

10:10

Tobias Dilworth/ Development of an Integrated Environmental DNA Sample Preparation Platform to Enable Autonomous Analysis Capabilities (Johns Hopkins University Applied Physics Laboratory)

10:30

Kristina Zudock & Hayley DeHart/ Validation and refinement of rapid eDNA sequencing methods and analyses for marine mammal observation networks in polar oceans (Johns Hopkins University Applied Physics Laboratory)

10:50

Kevan Yamahara/ Development of a new autonomous eDNA sampler based on 25 years of deployments and instrument optimization (Monterey Bay Aquarium Research Institute)

11:10

11:30

Stephen Spear/ Evaluating potential of robotic eDNA samplers for detecting onset of Grass Carp spawning events (USGS, Upper Midwest Environmental Sciences Center)

David Baisch/ The NABIT: Nucleic acid barcode identification tool (Conservation X Labs)

11:50 - 12:00

Break

12:00–12:30

Poster Blitz 3

Chair: Cathy Richter Chat Monitor: Nicole Miller

12:00

Tamara Schenekar/ Optimizing eDNA capture from Southern African waterholes (University of Graz, Austria)

12:05

Brandon Sansom/ Development and evaluation of one-dimensional eDNA transport models for freshwater mussels (USGS, Columbia Environmental Research Center)

12:10

12:15

Adam Perez/ Environmental DNA CRISPR biosensors for the detection of wildlife diseases and invasive species (Cherokee Nation System Solutions)

Madeleine Malmfeldt/ In the Beginning: Using eDNA Traces in Terrestrial and Subterranean Systems for Key Environmental Recon and Intel Purposes (Environmental Laboratory, US Army Engineer Research & Development Center)

12:20 –12:30

Poster Blitz Question & Answer

12:30-13:20

13:20-13:30

13:30

Concluding Plenary

Chair: Maggie Hunter

Susanna Theroux/ Advancing eDNA method adoption for bioassessment and biomonitoring (Southern California Coastal Water Research Project)

Wrap-up

Adjourn

6eDTEW Connection Directions and Info

● 6eDTEW will be hosted on an Cisco WebEx platform.

● Each day will have a different WebEx link for joining (see below). Please clink on the link (or cut and paste the link into a browser) each time you need to join or rejoin the workshop on the associated day.

● You may be asked to download and install the WebEx software the first time you attempt to join the workshop, so allow for some time to do that.

● You may or may not be asked to provide the meeting number and/or password for that day’s workshop when you join the meeting.

● There should be three options for the audio feed:

o Computer audio (we assume this will be a free option for all participants)

o Call a US toll-free number (we’re not sure whether this will be toll free for anyone outside the US. Seems unlikely)

o Have the meeting or WebEx call you (when we conducted eDTEWs in the past using WebEx this was a free option for everyone regardless of which country the attendee was situated in. We hope that is the case this time as well).

6eDTEW - Day 1

Tuesday, Jan 24, 2023

Workshop Link:

https://usace1.webex.com/usace1/j.php?MTID=m8f2297bea1a32fb838387c2a4162c5da

Meeting number: 2762 401 5896

Password: JAqmi8J2m*2

NOTE: 6th Annual eDNA Technical Exchange Workshop. WebEx connection opens early at 10:00 AM EST (GMT-5) and 6eDTEW begins later at 12:00 PM EST. Feel free to login (and logout) early to check your connection.

Join by video system

Dial 27624015896@usace1.webex.com

You can also dial 207.182.190.20 and enter your meeting number.

Join by phone

+1-844-800-2712 US Toll Free

Access code: 276 240 15896

6eDTEW - Day 2

Wednesday, Jan 25, 2023

Workshop Link:

https://usace1.webex.com/usace1/j.php?MTID=mddf27fb0d1d9f3541e5d4610fc593710

Meeting number: 2762 913 4410

Password: YWvCN9dJ$33

NOTE: Day 2, 6th Annual eDNA Technical Exchange Workshop. WebEx connection opens early at 09:00 AM EST (GMT-5) and 6eDTEW begins later at 10:00 AM EST. Feel free to login (and logout) early to check your connection.

Join by video system

Dial 27629134410@usace1.webex.com

You can also dial 207.182.190.20 and enter your meeting number.

Join by phone

+1-844-800-2712 US Toll Free

Access code: 276 291 34410

6eDTEW - Day 3

Thursday, Jan 26, 2023

Workshop Link:

https://usace1.webex.com/usace1/j.php?MTID=md6b7867ba5af9cdcbf40d5180304456f

Meeting number: 2764 538 8331

Password: buGAjA8c*74

NOTE: Day 3, 6th Annual eDNA Technical Exchange Workshop. WebEx connection opens early at 09:00 AM EST (GMT-5) and 6eDTEW begins later at 10:00 AM EST. Feel free to login (and logout) early to check your connection.

Join by video system

Dial 27645388331@usace1.webex.com

You can also dial 207.182.190.20 and enter your meeting number.

Join by phone

+1-844-800-2712 US Toll Free

Access code: 276 453 88331

! During the presentation sessions, everyone will have audio muted, unless you are presenting or selected by a session moderator to ask a question (use the “raise hand” feature).

! During the open discussion sessions, (use the “raise hand” feature) to alert a session monitor to unmute you.

! Please do not turn on your personal video feed, unless you are speaking (it is optional then).

! Each day’s WebEx session will open 1-1.5 hours early to allow attendees to test their connection. You may log in early, check you connection, logout, and then log back in shortly before the first session of the day begins (or just stay logged in if you like).