13th Annual FLIM Workshop in the Biomedical Sciences

Join us at the 13th Annual FLIM Workshop in the Biomedical Sciences on Thursday + Friday, October 31 - November 1, 2019 in Bethesda, MD. The event is organized by Boston Electronics Corporation in Brookline, MA and Becker & Hickl GmbH in Berlin, Germany. This free 2-day workshop covers many facets of Fluorescence Lifetime Imaging applications in the biomedical sciences on the following topics:

• FRET-FLIM

• Metabolic Imaging (NADH Imaging)

• Clinical FLIM Applications

• Fast FLIM

• Fluorescence Correlation Spectroscopy (FCS)

• Fluorescence Anisotropy

• Transient Fluorescence Lifetime Effects

• Measurement of Molecular-Environment Parameters

• Oxygen Concentration in Cells and Tissue

• PLIM (Phosphorescence Lifetime Imaging)
  
  

Agenda

Thursday, October 31, 2019

07:30 AM - 09:00 AM
Breakfast

09:00 AM - 09:45 AM
Wolfgang Becker, Becker & Hickl GmbH
“The Multi-Dimensional World of TCSPC FLIM”

09:45 AM - 10:15 AM
Kayvan Samimi, Mordgridge Institute for Research
"Multiphoton FLIM of visual cycle retinoids in mouse retina and human PSC-derived photoreceptor cells"

10:15 AM - 10:45 AM
Artem Pliss, SUNY Buffalo
“Dynamic cellular architecture studied by fluorescence lifetime imaging”

10.45 AM - 11.00 AM
Coffee Break

11:00 AM - 11:30 AM
Ahmed Heikal, University of Minnesota
"New developments on FRET-based environmental sensing using integrated fluorescence methods."

11:30 AM - 12:00 PM
Osman Bilsel, UMass Medical School
“The unfolded state of proteins viewed with time-resolved FRET, unnatural amino acids and microfluidic mixing”

12.00 PM - 12.30 PM
Hauke Studier, Becker & Hickl GmbH
"Multiphoton FLIM is Gaining Ground as a Clinical Tool"

12:30 PM - 01:30 PM
Lunch Break (The Bethesdan Hotel)

01:30 PM - 02:00 PM
Dan Needleman, Harvard University
"Metabolic Imaging of Mammalian Oocytes and Embryos with FLIM"

02:00 PM - 02:30 PM
Michael Evers, Massachusetts General Hospital, Harvard Medical School
"Enhanced metabolic quantification of adipose cells and tissue by two-photon excited NADH FLIM"

02:30 PM - 03:00 PM
Patrick Schäfer, Children's Hospital of Philadelphia
"NADH FLIM in assessing skeletal muscle energy metabolism of mitochondrial mutant mice"

03:00 PM - 03:15 PM
Coffee Break

03:15 PM - 03:45 PM
Ammasi Periasamy, UVA Keck Center for Cellular Imaging
"Evaluation of Fluorescence Lifetime Redox Ratio (FLIRR) in Living Prostate Cancer Cells"

03:45 PM - 04:30 PM
Jay Knutson, NIH / NHLBI
“FLIM studies of heterogeneous oxygen consumption and NADH metabolism in cells and mitochondria”

04:30 PM - 05:00 PM
Alex Walsh, Texas A&M University
"Fluorescence lifetime imaging of NADH and FAD reveals metabolic heterogeneity in immune cells"

06:00 PM
Dinner
Rock Bottom Brewery, 7900 Norfolk Ave, Bethesda, MD 20814

Friday, November 1, 2019

07:30 AM - 09:00 AM
Breakfast

09:00 AM - 09:45 AM
Steve Vogel, NIH / NIAAA
"Using FRET spectroscopy and FCS to study conformational changes associated with Venus-tagged CaMKII activation and T-site interactions"

09:45 AM - 10:15 AM
Gary Yellen, Harvard Medical School
"Neuronal metabolic responses to stimulation analyzed with fast FLIM of metabolic biosensors"

10:15 AM - 10:45 AM
Dave Lovinger, NIH / NIAAA
"Measuring Intracellular Signaling In Vivo with Time-Correlated Single Photon Counting Fiber Photometry"

10.45 AM - 11.00 AM
Coffee Break

11:00 AM - 11:30 AM
Horst Wallrabe, UVA Keck Center for Cellular Imaging
"Autofluorescent FLIM data analysis – multi exponential or incomplete fitting?"

11:30 AM - 12:15 PM
Wolgang Becker, Becker & Hickl GmbH
"Metabolic Imaging by Simultaneous Measurement of NADH and FAD"

12:15 PM - 01:30 PM
Lunch Break (The Bethesdan Hotel)

Accommodation

The Bethesdan (DoubleTree by Hilton), 8120 Wisconsin Ave, Bethesda, MD 20814

Follow link to book at special workshop rate.

Workshop rate available until Thursday, 10/14/2019

This workshop is sponsored by